Patient safety culture reflects the persistence and behavior of hospital employees in terms of safety. The medical institutions in Taiwan mostly take the annual healthcare quality and PSC for hospitals announced by Ministry of Health and Welfare as a way to increase healthcare quality and patient safety. A hospital used some mixed strategies to establish employees’ patient safety culture. By reviewing the results of patient safety culture surveys reported by employees, we found that the results confirmed the effectiveness of the measures. Through forward-looking training strategies, a patient safety culture can be effectively developed.