P-groups格式於HLA-DPB1分型中的應用

Application of the P-groups Format in HLA-DPB1 Typing

本研究探討P groups代碼格式在人類白血球抗原（HLA） DPB1分型檢測中的應用，並比較低中解析度-反向序列特異性寡核苷酸法與高解析度──序列定序方法的轉換準確性與一致性。結果顯示，在低中解析度方法中，仍存在部分無法解決的型別歧異性。然而，在高解析度方法中，179例樣本中有168例（93.9%）可透過P groups代碼轉換選定最適合的型別，並部分解決型別歧異問題，顯示P groups格式在高解析度HLA分型中的應用潛力。綜合而言，P groups代碼可作為HLA-DPB1分型輔助工具，特別適用於序列定序方法，未來可進一步探討其在臨床應用與移植配對中的價值。

This study explores the application of the P groups coding format in HLA-DPB1 typing and evaluates the conversion accuracy and consistency between the low-to-moderate resolution reverse Sequence-Specific Oligonucleotide (r-SSO) technique and the high-resolution Sequence-Based Typing (SBT) technique. The results show that the r-SSO method still has unresolved allele ambiguities. However, in the SBT method, 168 out of 179 samples (93.9%) could be assigned the most suitable allele through P groups conversion, partially resolving allele ambiguities. These findings demonstrate the potential of the P groups format in high-resolution HLA typing. Overall, the P groups coding system can serve as an auxiliary tool for HLA-DPB1 typing, particularly in SBT, and its clinical applications and implications for transplantation matching warrant further investigation.