使用二氧化碳飛梭雷射促進熊果素之經皮吸收

Enhancing skin permeation of arbutin with fractional CO2 laser

熊果素(arbutin)是一種從天然植物中萃取出來的成分，廣泛應用於化妝品中，並被認為是安全有效的美白成分之一。本研究以二氧化碳飛梭雷射(fractional CO2 laser)當作促進試驗藥物的工具，來證實是否能有效提升熊果素的經皮吸收能力，同時找出可利用於臨床試驗的最佳雷射能量。首先將試驗皮膚由不同能量的二氧化碳飛梭雷射施打後，再接續分析六個時間點之熊果素穿透濃度，之後計算其穿透速率(flux)及促進倍率(enhancement ratio, ER)，來比較不同雷射能量對促進熊果素經皮吸收能力之影響。為了瞭解二氧化碳飛梭雷射可能促進試驗藥物的機轉，則在二氧化碳飛梭雷射處理試驗皮膚後，再利用掃描式電子顯微鏡(scanning electron microscope, SEM)來觀察試驗皮膚結構上的變化。結果顯示，經由二氧化碳飛梭雷射處理的組別，與未處理的組別比較下，其熊果素促進倍率為21~63倍。二氧化碳飛梭雷射會在皮膚上產生微小孔洞，並破壞角質層的結構，可能是增加熊果素穿透能力的因素之一。本研究證實，二氧化碳飛梭雷射可顯著提升熊果素之經皮吸收能力，而最適雷射之能量設定為9瓦。

Arbutin, a skin-whitening ingredient extracted from natural plants, is widely utilized in cosmetics and is considered a safe and effective whitening ingredient. This study employs a fractional CO2 laser to enhance the transdermal delivery of arbutin to evaluate its potential to enhance arbutin's penetration and determine the optimal laser energy for clinical trials. Skin samples penetration. This study confirms that the fractional CO2 laser significantly enhances the transdermal were initially treated with varying energy levels of the fractional CO2 laser to facilitate arbutin delivery of arbutin, as evidenced by the analysis of arbutin penetration concentrations at six different time intervals. The penetration rate（flux）and enhancement ratio（ER）were then calculated to assess the impact of varying laser energies on arbutin transdermal absorption. A scanning electron microscope（SEM）was employed to examine structural changes in the treated skin, revealing that the fractional CO2 laser creates microchannels and disrupts the stratum corneum, enhancing arbutin absorption. Results indicate that the enhancement ratio of arbutin in the fractional CO2 laser-treated group was 2 to 63 times higher than in the untreated group. The optimal laser energy setting determined for effective treatment was 9 watts.