應用於蔬菜檢測的農藥免疫快篩平台：菲克利快篩試劑的開發與應用

Pesticide Immunoassay Rapid Screening Platform Applied in Vegetable Testing: Development and Application of Hexaconazole Rapid Screening Assay

隨著人們生活品質提高，食品安全需求日益重要。農業科技發展帶來農藥使用增加，引起農產品農藥殘留問題，成為全球議題。質譜法雖為檢測黃金標準，但設備昂貴且操作繁瑣，耗時費力。因此，我們計劃開發快篩試劑，使用具成本低、操作方便、即時結果、肉眼判讀等優點的側向流動免疫分析平台。此平台廣泛應用於農業、生醫、環境科學等領域。本研究針對菲克利農藥，提升偵測靈敏度，優化快篩試劑，以達到蔬果農藥殘留的最小容許量。結果顯示開發之快篩試劑具有快速、準確、操作簡易、靈敏度高等特點，檢測靈敏度達0. ppm，有助於加速農藥殘留檢測，保障食品安全。

As the quality of life improves, the importance of food safety grows significantly. The progress in agricultural technology has led to increased pesticide use, raising concerns about pesticide residues in agricultural products - a global issue. While mass spectrometry is the preferred method for detection, its costly equipment and complex operation make it time-consuming and labor-intensive. Hence, our focus is on developing rapid screening assays using lateral flow immunoassay (LFIA) technology. LFIA offers advantages such as affordability, ease of use, immediate results, and visual interpretation, widely applied across agriculture, biomedicine, environmental science, and other domains. Our study concentrates on the pesticide hexaconazole, aiming to boost detection sensitivity and optimize rapid screening assays to meet minimum allowable pesticide residue levels in fruits and vegetables. Our rapid screening assays boast characteristics like speed, accuracy, ease of use, affordability, and high sensitivity, with a detection sensitivity of 0.1 ppm. This contributes to swift pesticide residue detection, thereby ensuring food safety.