以全自動血球分析儀及流式細胞儀評估造血幹細胞收集前白血球數及周邊血造血幹細胞數與收集後成品幹細胞總量相關性之探討

Use automatic blood cell analyzer and flow cytometer to evaluate the correlation between the number of white blood cells and peripheral blood hematopoietic stem cells before collection and total stem cell count of product

周邊血幹細胞移植為一種細胞治療法，將捐者骨髓內造血幹細胞驅動至周邊收集並處理後給予受贈者輸注，而收集時機十分重要，故我們以全自動血液分析儀及流式細胞儀檢測驅動後周邊血之白血球數及造血幹細胞數，前者細胞數與成品幹細胞總量相關性較低，後者較高；而兩儀器所檢測的白血球數之相關性較幹細胞數高，但後者仍有一定的表現；捐者年齡及來源為自體或異體與成品細胞數關係小，若希望收集量達標，周邊血幹細胞濃度應大於19 cells/μl。

Peripheral blood stem cell transplantation is a stem cell therapy. To collect peripheral blood stem cells, donor needs to inject granulocyte colony stimulating factor, an immunostimulant to mobilize the blood stem cells in bone marrow to peripheral blood several days before and during the harvest day.
Because the collection timing is very important, we use automatic hematology analyzer and flow cytometry to analyze the cell numbers of white blood cell (WBC) and hematopoietic stem cell before harvest. The correlation coefficient between hematopoietic stem cell counts and total stem cell counts of final product is higher than the correlation coefficient between WBC counts and total stem cell counts of final product. The correlation between the numbers of WBC detected by the two instruments is higher than the numbers of hematopoietic stem cell, but the two instruments still can be used to evaluate the clinical collection timing. The age of the donor and whether the source is autologous or allogeneic have little effect on the cell counts of product. If we want the total stem cell counts of final product to meet the target goal, the concentration of peripheral blood stem cells after mobilizing should be higher than 19 cells /μl.