利用超薄片法及冰蝕法印證煙草原生質體的超微構造

Complementary Studies of Tobacco Protoplast With Freeze-Fracture and Thin Section Techniques

振盪培養細胞經以酵素法處理可得圓形而無壁的原生質體，經切片或冰蝕法處理可得相互印證，內質網發達板囊常有3～10層，又高爾基體常在其附近出現，不同性質的油滴分別以單一或聚集存在，粒線體形狀穩定而色素體的變化較大，大部分的原生質體內都有許多小液泡充塞著而大液泡消失，大而圓的細胞核位於原生質體的中央，原生質體經誘導處理可產生一含兩個以上的核的原生質體，原生質體是研究細胞壁再生的好材料。

Tobacco protoplasts enzymatically isolated from suspension culture were studied . Isolated protoplasts showed intact plasma membrane surrounded the outer surface, but sometimes membrane with breb was also observed. Endoplasmic reticulum was abundant and 3 to 10 layers of cisternae in parallel arrangement often occurred. Invaginations of cytoplasmic membrane with some organelles in it were al so found. Oil drops prominently occurred in isolated protoplasts with different characters in staining properties and structure. The central and large vacuoles disappeared and small vacuoles tremendously increased in number. Nucleus located in the center of protoplasts with a distinct nucleolus. Annuli on the surface of nuclear envelop were numerous (35/um2). Plastids were different in shape and size. Structural patterns of plastids are more diverse than that of mitochondria. Stereo pairs showed the three dimensional structures of protoplasts were all identical to that observed in thin sections.