香砂六君子湯於厭食症小鼠模式之腸道菌相分析

Exploring the Impact of Xiangshaliujunzi Decoction in an Anorexia Mouse Model of Intestinal Microbiota

厭食症是一種飲食失調症，目前對其病因的了解不足，未能有效地治療方案。最新研究揭示了厭食症與代謝之間具有相關性，本研究將致力於探討中草藥香砂六君子湯對於厭食症潛在地腸道菌相調控機制。香砂六君子湯由人參、半夏、白朮、茯苓、木香、陳皮、砂仁、甘草、生薑組成復方。可用來治療針對腸胃相關疾病。本研究利用分離誘導厭食症小鼠模式，餵食香砂六君子湯0.1或1克／公斤連續14天後觀察體重與飲食變化量，並採集糞便定序分析16S腸道菌相，結果數據採用微陣列與Tax4Fun2等多種統計與分析系統進行剖析。本研究發現香砂六君子湯具有潛在治療厭食症藥理作用，並且調整腸道菌相達到臨床症狀改善的作用，未來也可以應用於不同腸道相關疾病的治療潛力。

Anorexia is an eating disorder. It's etiology is currently poorly understood and there are no effective treatment options. Recently, research has revealed the correlation between anorexia and metabolism. This study focus on exploring the potential regulation mechanism of gut microbiota in anorexia with a Chinese herbal medicine, Xiangshaliujunzi Decoction (XLD). XLD is composed of ginseng, pinellia, atractylodes, poria, muxiang, chenpi, amomum, licorice, and ginger. It can be used to treat gastrointestinal related diseases. In this study, we established a separation-induced anorexia model and fed participants with 0.1 or 1 g/kg of XLD for 14 consecutive days to observe changes in body weight and diet. Subsequently, we sampled feces to sequence and analyze the 16S intestinal microbiota. Additionally, sequencing data were analyzed by a multiple bioinformatics system. This study found that XLD has a potential pharmacological effect on anorexia, and adjusting the intestinal microbiota can achieve pathological improvement. XLD has therapeutic potential for different intestinal-related diseases.