食品中硼酸之檢驗方法探討   全文下載

Evaluation of the Test Method for Boric Acid in Food

現行衛生福利部食品藥物管理署（下稱食藥署）公開之「食品中硼酸之檢驗方法（TFDAA0086.00）」，係利用樣品經萃取後，與衍生試劑反應呈色，以分光光度計檢測，考量其使用毒性較高之氯仿作為衍生化之萃取溶劑，因此本研究評估使用其他溶劑系統之可行性，並以紅麴酒糟、紫米及湯圓三種基質進行專一性、精密度及定量極限之評估測試。本研究結果顯示以正己烷／乙酸乙酯（1:1, v/v）混合溶液作為萃取溶劑，其平均回收率介於92.5-104.8%，顯示可作為替代之萃取溶劑；以紅色色素、紅麴酒糟、紫米及湯圓進行專一性評估，結果顯示均無顏色干擾之情形。另準確度及精密度試驗，結果顯示，於基質中添加硼酸300及600 mg/kg，其同日內之平均回收率分別為94.9-102.7%及97.0-101.9%，變異係數分別為2.8-6.7%及0.7-3.1%；於異日間之變異係數則分別為3.3-5.6%及1.2-4.8%，皆符合食藥署食品化學檢驗方法之確效規範，本檢驗方法之定量極限為300 mg/kg。

This study evaluated the feasibility of using less hazardous solvent system as a substitute for chloroform during the derivatization process in the “Method of Test for Boric Acid in Foods (TFDAA0086.00)” published on May 24, 2020. The red-colored food matrices (red yeast rice, purple rice and glutinous rice ball) were selected as sample to evaluate the specificity, precision and limit of quantitation of the method in different solvent systems. The recoveries of boric acid were 92.5-104.8% when using the mixture solution of n-hexane/ethyl acetate mixture (1:1, v/v) as the extraction solvent system. The results showed this solvent system could be a suitable replacement of chloroform. The modified method for boric acid in foods was validated in red colorants and the red-colored foods. The results showed there was no interference from the red substances. The accuracy and precision tests were conducted by spiking 300 mg/kg and 600 mg/kg boric acid into matrices described above, and the results showed that the intra-day average recoveries were 94.9-102.7% and 97.0-101.9% for 300 mg/kg and 600 mg/kg spiked sample, respectively. Their coefficients of variation were 2.8-6.7% and 0.7-3.1%, respectively. The inter-day coefficients of variation were 3.3-5.6% and 1.2-4.8%, respectively. The limit of quantification was 300 mg/kg.