柬埔寨餘甘子萃取物抗發炎效果之研究

Anti-inflammatory Effect Extract of Emblica Fruit from Cambodia

本文以柬埔寨餘甘子（Phyllanthus emblica L.）萃取物為材料，研究其對巨噬細胞株的抗發炎效果。應用體外培養的細胞株，以三種促炎因子，包含一氧化氮（nitric oxide, NO）、白介素6（interleukin 6, IL-6）、腫瘤壞死因子-α（tumor necrosis factor-alpha, TNF-α）進行分析，並進行細胞毒性（microculture tetrazolium assay, MTT assay）分析。結果顯示，柬埔寨餘甘子萃取物具顯著抑制由細菌脂多醣（lipopolysaccharide, LPS）所誘導巨噬細胞株產生大量的NO與IL-6之發炎反應。對於NO，在細胞培養液中添加5％萃取物的發炎反應抑制率為31.9％（正控制組之68.1%±5.1%），添加10％萃取物的抑制率為45.8％（正控制組之54.2%±5.6%）。對於IL-6，添加5％的抑制率為33.9％（正控制組之66.1%±9.7%），添加10％的抑制率為44.0％（正控制組之56.0%±1.3%）。但對TNF-α則無顯著抑制作用。同時，在本研究之測試濃度下未測得顯著細胞毒性。綜上所述，柬埔寨餘甘子萃取物在體外培養的細胞模式中，能有效抑制LPS所刺激誘導NO與IL-6的發炎反應。

This study used emblica fruit (Phyllanthus emblica L.) from Cambodia solution as the material to explore its anti-inflammatory response effect on cells. Using cell lines cultured in vitro to analyze nitric oxide（NO）, interleukin-6（IL-6）, tumor necrosis factor-α（TNF-α）that cause inflammation and processed cytotoxicity（microculture tetrazolium assay, MTT assay）analysis. The results showed that the micronized emblica solution significantly inhibited the inflammatory response of NO and IL-6 induced by the bacterial lipopolysaccharide（LPS）activated macrophage cell line. For NO, the inhibition rate of adding 5% was 31.9％（68.1%±5.1%）, and the inhibition rate of adding 10% was 45.8％（54.2%±5.6%）. For IL-6, the inhibition rate of adding 5% was 33.9％（66.1%±9.7%）, and the inhibition rate of adding 10% was 44.0％（56.0%±1.3%）. But it has no inhibitory effect on TNF-α. Meanwhile, no cytotoxicity was detected at the tested concentrations. In summary, the micronized emblica solution has effectively inhibited the inflammatory response of NO and IL-6 mediated by LPS stimulation in cells cultured in vitro.