唐棉嵌紋病毒（gomphocarpus mosaic virus, GoMV）特性分析

唐棉 （Gomphocarpus spp. syn. Asclepias spp.） 為夾竹桃科 （family Apocynaceae） 蘿藦亞科 （subfamily Asclepiadoideae） 多年生草本花卉作物。Gomphocarpus mosaic virus （GoMV）感染引起葉片嵌紋、葉面凹凸、葉片窄小、葉緣波浪狀等病徵的植株經電子顯微鏡鏡檢，可觀察到大小約750-850 x 12 nm之長絲狀病毒顆粒；病組織超薄切片亦可觀察到細胞內有典型potyvirus 內含體。單離的GoMV病毒株機械接種至9科24種的指示植物，結果顯示夾竹桃科 （Apocenaceae）、藜科 （Chenopodiaceae） 與茄科 （Solanaceae） 中共有14種供試植物會被感染。其中9種供試植物之病徵表現屬於局部單斑寄主；5種屬於系統性寄主，包括原寄主唐棉。純化之病毒顆粒懸浮液以SDS-PAGE分析後，可觀察到大小約30 kDa之鞘蛋白條帶。純化GoMV製備病毒抗血清，於雙向免疫擴散反應中，與純化之病毒懸浮液產生白色沉澱帶且抗血清之力價達1/2。以間接式酵素連結免疫吸附分析法 （indirect enzyme-linked immunosorbent assay, indirect-ELISA） 與西方轉漬反應 （western blotting），該抗血清與接種GoMV之唐棉病葉粗汁液均產生正反應。

Gomphocarpus physocarpus, commonly known as balloon plant or swan plant, is a perennial herbaceous ornamental crop of the subfamily Asclepiadoideae, family Apocynaceae. Gomphocarpus mosaic virus (GoMV), a new and emerging potyvirus, infects Gomphocapus spp. causing symptoms of mosaic, mottle and crinkling on leaves. Electron microscopic examination revealed the presence of filamentous virus-like particles about 750-850 x 12 nm in crude sap and typical potyviral pinwheel and laminated inclusion bodies in ultrathin sections of infected tissues. A GoMV isolate was mechanically inoculated to 24 plant species belonging to nine families to determine its host range. Fourteen species of plants out of 24 tested were affected. SDS-PAGE analyses revealed that GoMV contains a single viral coat protein subunit of a molecular weight about 30 kDa. Specific antiserum against the GoMV was produced by immunizing New Zealand white rabbit with purified virus particles. The specific antiserum reacted positively to GoMV in all tests using SDS-agar gel double diffusion assays, western blotting, and indirect enzyme-linked immunosorbent assay (ELISA).