傳統與現代昆蟲鑑定技術的結合──以蘋果蠹蛾（鱗翅目：捲葉蛾科）為例

"A combination of traditional and modern techniques for insect identification - using the codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), as an example"

外國農產品進口所最要防範的，就是可能藏匿其中的有害生物（如昆蟲、蟎蜱、線蟲或植物病原菌等等）的入侵，進而危害本地的農業及生態環境。為確保我國農業自身的安全，避免有害生物的境外入侵，此一把關的重責大任即是由行政院農業委員會所屬的動植物防疫檢疫局（簡稱檢疫局）負擔著。

"In this work, we combined traditional and modern techniques, i.e., morphological and species-specific DNA markers, respectively, to identify the codling moth, Cydia pomonella L. According to a published article, morphological characters, mainly the style of the proleg crochets and the number and distribution of setae, etc., were applied for identification of codling moth larvae. The rationale of sequence characterized amplified regions (SCARs) was adopted to develop DNA markers for identification of codling moths, and we obtained 5 pairs of species-specific PCR primers for this purpose. By applying these 2 methods, we successfully identified 2 codling moth specimens which were discovered in 2 batches of apples imported from the US in the course of quarantine inspections by the Kaohsiung Division of the Bureau of Animal and Plant Health Inspection and Quarantine (BAPHIQ) on November 6 and 12, 2002, respectively."