Catheter- associated urinary tract infection (CAUTI) is the most common issue in medical institutions . It not only increases hospital stays and medical costs, but also increases the severity of patients’illnesses. From January to June 2015, the average incidence of catheter-associated urinary tract infection was ranked first in the acute ward of hospitals. The factors we found that may cause this high incidence included: the physicians failed to actively assess whether the catheter needs to be removed, the staff failed to comply with aseptic technique for performing indwelling catheter technology, didn’t execute proper hand hygiene, didn’t execute the daily examination and care of catheter, didn’t keep the appropriate distance between the opening of urine collection bags and the ground, and the lack of routine examination for catheterization and appropriate urine collection barrels. We established the project team in order to analyze and explore the rationale. From 2015/8/1 to 2016/9/30, we introduced the “CAUTI Bundle Care” mode from the Joint Commission of Taiwan. This was done through holding in-service education, introducing and passing on hand hygiene routines, using nursing innovation supplies such as urine collection bags, regular examination of the catheterization, and so on. The sixmonth evaluation indicated that the average incidence of catheter-associated urinary tract infection decreased from 0.84 ‰ to 0.15 ‰, the effect maintained 0.00‰ for 6 months, and the average urinary catheter-days decreased from 8.7 to 4.1. Overall, the project resulted in excellent improvements. The quality of care of clinically relevant units and patients can be further improved by sharing expectations