Introduction: Physical inactivity is a behavioral risk factor of elderly’s health. Walking behavior has been recognized as a suggested form of physical activity for older adults. Evidence shows that environment is a determinants of older adults’ walking behavior. According to Ecological model, this study aims to review the literature between environment and older adults’ walking, including objectively-measured built environment and perceived environment. Methods: This study using systematic review to better understand the associations of objectively-measured and subjectively environmental factors with older adults’ objective and subjective walking behavior, in order to provide future suggestions. Results: Among 19 included studies, 17 were quantitative studies using cross-sectional design. Overall, included studies used walkability as core concept of environmental factors. The results showed that density, safety, land use mix and friendly environment is positively associated with different types of walking behavior among older adults. Besides, more studies using questionnaire to assess older adults’ walking behavior, fewer studies have used objective measures. Conclusion: First, more qualitative studies are still needed to better understand the perceived barriers and facilitators of walking behavior among older adults. In addition, self-selection should be included as a key confounder to clarify the influence of environment on walking behavior. Moreover, prospective or quasi experimental design not only can minimize the self-selection bias (active individuals tend to choose neighborhoods conductive to physical activity), as well as improving evidence related to causality. Third, studies can be beneficial to utilize both objective and subjective measures of walking behavior for accuracy. Fourth, the definition of buffer zone varied in different studies and thus it is difficult to define the neighborhood environment of older adults. Fifth, for considering the limitation of environmental measures, Walk score® website is a novel tool for measuring walkability. However, the validity of Walk score® website should be further tested in Asian countries. Finally, considering the different culture, residential density and infrastructure between Asian and Western Countries, it is important to further provide the indicators of Walkability for Asian countries.