| 中文摘要 |
期刊連結：http://www.gouthyperuricemia.org
Objective: Gouty arthritis is a sharp inflammatory arthritis induced by monosodium urate (MSU) crystals. Previous studies indicate that the T cell immunoglobulin and mucin domain molecule 4 (TIM-4) is an adhesive molecule which binds to phosphatidylserine (PS) to mediate phagocytosis of apoptotic cells by macrophages, which also regulate T cell activation and increase proinflammatory cytokine expression. This study aims to investigate whether TIM-4 is involved in MSU-induced gouty inflammation.
Methods: MSU suspensions were injected into ankle joints or foot pads of TIM-4 knock-out (KO) and wild type (WT) mice to induce acute gout. Ankle swelling or paw edema were measured at different time points with digital caliper. MSU suspensions were injected into peritoneal cavity or air pouch. Peritoneal cavity or air pouch exudate cells were then harvested after 3 or 6 hours and the infiltrated macrophages and neutrophils were counted by Fluorescence-Activated Cell Sorting (FACS).
Results: In the ankle joints model of MSU-induced gouty arthritis, ankle joint swelling index in TIM-4 KO mice was not significantly different compared with WT mice. Consistent with the ankle joints model, the paw swelling index also had no difference in TIM-4 KO mice compared with WT controls. Additionally, there were no dramatic changes in total number of peritoneal cavity or air pouch exudate cells, neutrophils influxes and infiltrated macrophages between KO and WT mice.
Conclusion: TIM-4 may not play a crucial role in the development of MSU-induced gouty inflammation. |
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