Ebstein’s anomaly with Wolff-Parkinson-White (WPW) syndrome is a rare congenital heart disease with malformation of the tricuspid valve. Considering the high risk for sudden death, the catheter ablation for WPW syndrome or surgical intervention or repair of tricuspid valve replacement is usually suggested to the patient to improve the survival rate. In this report, we present a 25-year-old male who suffered from frequent palpitation was diagnosed with Ebstein’s anomaly with Wolff-Parkinson-White (WPW) and underwent a catheter ablation. But, the WPW syndrome was reoccurred in three hours after the catheter ablation. Based on the case, this article discusses the pathophysiology, medical treatment, and clinical care of Ebstein’s anomaly with WPW syndrome and the possible mechanism of reoccurrence for WPW syndrome after a successful catheter ablation.