牡丹皮、天門冬精華液在減輕女性臉部皮膚暗沉之研究

The Lotion Containing the Extract of Paeonia Suffruticosa and Asparagus Cochinchinensis Attenuated Skin Pigmentation in Women: A Pilot Study

前言：傳統中醫認為當人體長期處於體液缺乏（陰虛），血瘀和血熱會造成 發炎後色素沉著。臨床中醫通常使用滋陰中藥，如天門冬來滋養人體體液及 皮膚，並利用活血化瘀中藥如牡丹皮來促進血液循環來治療發炎後色素沉著。 先前研究已證實天門冬和牡丹皮萃取物有效地抑制黑色素細胞的酪氨酸酶活 性，但尚未有相關實證醫學研究來探討其皮膚美白功效。本研究目的在探討 含有天門冬及牡丹皮萃取物的精華液對女性發炎後色素沉著之改善效果。 方法：本研究是隨機單盲臨床試驗，共有 60 位年齡為 25 至 60 歲健康女性 受試者，其 Fitzpatrick 皮膚類型為 III 及 IV 型，將其隨機分為 3 組，0.5% 牡丹皮、天門冬精華液，2% 牡丹皮、天門冬精華液及未含牡丹皮、天門冬 精華液之安慰劑，每組各 20 人。受試者每日 2 次臉部清洗後塗抹樣品於皮 膚暗沉部位。之後在試驗前（第 0 週）及試驗後（第 8 週）經數位膚質檢測 儀（VISIA）評估臉部黑色素程度。 結果：在使用後的第 8 週，0.5% 及 2% 牡丹皮、天門冬精華液確實改善受 試者全臉的皮膚表淺斑點（p

Traditional Chinese Medicine (TCM), one form of CAM, has indicated that long term body fluid deficiency(津液缺乏), blood stasis(血瘀)and blood heat(血熱)can cause SHD. Clinical TCM usually uses the enrich yin(滋陰) herb, such as Asparagus cochinchinensis (Ac), to nourish the human body fluid and moisturize the skin for improving skin metabolism, resulting in skin whitening. In addition, TCM also uses the angiogenesis promoting herb, such as Paeonia suffruticosa (Ps), to cure SHD through promoting blood circulation to dispel blood stasis(活血化瘀). Previous studies have revealed that the extract of Ps and Ac can effectively inhibit tyrosinase activity in melanocytes, but the evidence base for its skin whitening effect has not been investigated yet. In the present study, The lotion containing the extract of Ps and Ac was used to attenuate skin pigmentation in female subjects through a pilot study. This is a randomized, placebo-controlled, single-blind clinical trial. Sixty female subjects aged between 25 and 60 were enrolled, and their Fitzpatrick skin types were type III to IV Asian skin with hyperpigmentation conditions. There are three randomized groups in this pilot study, including 0.5% and 2% lotion and placebo, with 20 subjects in each group. All subjects in each group were administered various test samples in facial pigmentation areas twice a day after cleaning. Before (0 week) and after (8 week) treatment, all subjects were non-invasively evaluated by VISIA®photography. The results indicated that there was a significant decrease in superficial spots on the entire face after using 0.5% and 2% lotion (p<0.05). In summary, The lotion can effectively improve the skin pigmentation of subjects and displays skin whitening effect, and we suggest that the lotion may be used as a skin whitening product in the cosmetic or pharmaceutical industry in the future.