椪柑樹被毒素病傳染後其組織內多酚類與核酸增加情形之研究

Studies on the Increase of Nucleic Acid and Polyphenol in the Tissue of Virus Infeded Ponkan Trees

1.利用電光光度計，可似測出椪柑葉片組織內所含有多酚類之情況。濾光片Klett No. 42所示讀數較Klett No. 54為高應用上較為適合，其測未染病之實生苗所示波長之範圍為90.8±l.98mu，超此範圍，即有染病之嫌。用人工接種者所示之範圍為200.6±20.43mu。2.椪柑枝條木質部多酚類測定，在電光光度計上所示讀數之範圍較葉片為高，未染病之實生苗所示讀數為143.9±3.74mu，即木質部讀數必須超此數，始可視為有病嫌之植株。人工接種植株之讀數範圍為310.8 ±14.98mu。3.利用電光分光光度計測椪柑葉片及木質部多酚其最大光波吸收之範圍，未染病之實生苗分佈在440～500mu之間，人工接種植株最大分佈之範圍葉片為460～510mu之間，木質部為450～510mu之間。4. 椪柑葉片組織內核酸之測定，係利用電光分光光度計，未染病實生苗，最大吸收範圍分佈在450～500mu之問，人工接種之植株最大吸收範圍分佈在470～520mu之間。

It is now known that Tristeza is the only virus disease transmitted by aphid vector and the control of this virus disease by using virus free budwood becomes a critical factor .in many areas where the citrus have got different mixtures of virus. The author has proved from several tests that the disease in Taiwan groves is a mixed type, so we need a rapid method determine the virus free citrus trees for controlling Tristeza in this The purpose of this test is to develop a rapid Colorimeter and Snectronhoto meter method for assaying the virus content which is based on the polyphenol and nucleic acid incrased in the tissue of virus infected trees. The results this test are summarized as follows: 1. The Photoelectric colorimeter is available for determining the polyphenol content of Ponkan leaf-tissue. It has been found in this test that the filter Klett No. 42 has more conspicuous readings than the filter Klett No. 54. The range of polyphenol content shown by the leaf-tissue of virus free seedlings is 90.8±1.9Smu., and the range shown by the artificial inoculation trees is 200.6±20.43mu. 2. The xylem-tissue test with Photoelectric Colorimeter (filter Klett No.42.) showed higher range of readings than the leaf-tissue test. There are 143.9±3.74mu in virus free seedlings, and 316.S±3,74mu in artificial inoculation trees. 3. The polyphenol test with Photoelectric Spectrophotometer in visible range showed maximum absorption differences between the virus free seedlings and virus inoculated trees. The virus free seedlings occurred in range of 440~500mu; and the virus artificial inoculation trees occurred in the range of 460~510mu. The xylem-tissue test with Photoelectric Spectrophotometer got the same result. 4. The Nucleic acid test with the Photoelectric Spectrophotometer on leaf-tissue of Ponkan, the maximum absorption of virus free seedlings occurred in the range of 450~500mu., and the virus artifical inoculation trees occurred in the range of 470~520mu.