Rhabditis線虫對洋菇生育之作用機構研究

Effect of a Nematode Rhabditis sp. Upon Mushroom Growing

Rhabditis sp.之繁殖最適溫度為25℃ 28℃及30℃次之。15℃以下及33℃以上時，其繁殖率甚低。在25℃環境下，接種5至1000雙於堆肥內，10於後即可繁殖到最高密度。而其繁殖數量與接種雙數多寡似無關係。在5℃至28℃間Rhabditis sp.之生存率最高。在0℃及40℃溫度下經6天後全部死亡。同時接種Rhabditis sp.及洋菇菌絲於堆肥時，在25℃環境下洋菇菌絲之生長勢極不良。但俟洋菇菌絲在堆肥等培養基內生長一段時期後丙接種Rhabditis sp.時，線蟲之繁殖較低，而對洋菇菌絲之生長似無多大影響。

The reproduction of the nematode Rhabditis sp. was best at 25℃ less so at 28℃ and 30℃. Little or no reproduction was observed at 15℃ and 33℃. while a higher population was occurred in the compost after 10 days or 40 days of inoculation at 25℃. The initial number of the nematode in inocula, 5 to 1000, did not affect the population reached after 10 days of cultivation. The mortality rate of Rhabditis sp. was 1.35 to 4.44% after 6 days at 5℃ to 28℃ and 100% at 0 and 40℃. The mycelial growth of mushroom in the compost, at 25℃ was very poor when Rhabditis sp. and mushroom mycelium were inoculated at the same time. However, the growth of mushroom mycelium in the compost, corn compost agar or corn compost solution was not affected if Rhabditis sp. was inoculated after the mushroom mycelium had started growing for several days.