紅花鶴頂蘭之無菌發芽

In vitro Seed Germination of Phaius tankervilliae

取紅花鶴頂蘭授粉後130天之成熟種子，於0.5%次氯酸鈉溶液中消毒15分鐘可提高發芽率，消毒後超音波振盪對發芽不利。發芽對基本鹽類的適應範圍極廣，但需含鈣較多之配方。Trypyone 2 g/l添加於1/4MS、VW及Hyponex（7N-2.6P-15.8K）配方可減輕褐化之程度和促進發育。若再添加各種有機添加物，除椰子汁50ml/l外，皆令褐化加劇，最好於播種後原球體形成時移植一次，以避免褐化。植物生長調節劑NAA對發芽並無助益，BA反有抑制作用。原球體移植後，較高濃度的adenine及枸椽酸會抑制根葉的生長，尤其培養基中含活性碳時更加明顯，顯示活性碳具強烈的吸附作用，改變培養基中植物本身所代謝植物生長調節劑之比例。

The effects of medium composition on seed germination and seedling development in vitro were studied in Phaius tankervilliae when seeds were harvested 130 days after pollination. The morphology and anatomy of seeds and germination process were also observed under light and scanning micrograph. Seeds sterilized with 0.5% NaOCl solution for 15 minutes could enhance germination percentage up to 63.6%. Tryptone 2g/l could promote and eliminate protocorm browning during embryo development under 1/4 MS, VW, and Hyponex (7N -2. 6P-15.8K) 2g/l basal salts with 2% sucrose. Medium without calcium (such as Thomale's GD1) and enriched with various kinds organic additives except 50ml/l coconut water would increase protocorm browning in embryogenesis of Phaius tankervilliae. Growth of seedlings were inhibited by benzyl adenine, adenine, citric acid and activated charcoal. Root formation and root growth were strongly inhibited in medium containing activated charcoal. It seems likely that activated charcoal strongly influence the balance of plant growth regulators in medium.