硝酸銀對蝴蝶蘭培植體增殖之影響

Effect of Silver Nitrate on Proliferation of Phalaenopsis Tissues in Vitro

取蝴蝶蘭原球體及分生幼株之短縮莖為培植體行組織培養繁殖時，最易增殖、葉次之、而根則不易產生擬原球體。培養基中加入抗乙烯作用劑硝酸銀0.025～0.1mM或培植體先浸泡硝酸銀0.1mM溶液2小時後培養，均可加速及增多擬原球體之形成，進而形成小植株，尤以葉片處理，破壞葉片角質層，使擬原球體自切口及葉表面多量長出，效果最佳。

The effect of ethylene antagonist silver nitrate on protocorm-like bodies formation in various organs of plantlet of phalaenopsis (P. Golden Emperor 'Sweet' and P. Snow Mist 'Large Sepal') was studied. Protocorm-like bodies formation was enhanced by 0.025~0.1mM silver nitrate in cultured media or presoaking the organs in 0.1mM silver nitrate solution for 2 hours. Protocorm-like bodies formed not only from wounding area, but also on the surface of leaf after soaking in 0.1mM silver nitrate for 2 hours. It seems likely that AgNO3 soaking also disturbed the covering cuticle layer which may be a barrier for protocorm formation from leaf surface. The proliferation rates of protocorm-like bodies from stem were higher than from leaves. There was no any regeneration from root explant in this experiment.