英文摘要 |
The effect of ethylene antagonist silver nitrate on protocorm-like bodies formation in various organs of plantlet of phalaenopsis (P. Golden Emperor 'Sweet' and P. Snow Mist 'Large Sepal') was studied. Protocorm-like bodies formation was enhanced by 0.025~0.1mM silver nitrate in cultured media or presoaking the organs in 0.1mM silver nitrate solution for 2 hours. Protocorm-like bodies formed not only from wounding area, but also on the surface of leaf after soaking in 0.1mM silver nitrate for 2 hours. It seems likely that AgNO3 soaking also disturbed the covering cuticle layer which may be a barrier for protocorm formation from leaf surface. The proliferation rates of protocorm-like bodies from stem were higher than from leaves. There was no any regeneration from root explant in this experiment. |