百合之組織培養（二）培養基成分、光週期及pH值對百合癒合組織生長、分化及葉綠素生成之影響

Studies on the Tissue Culture of Lilium longi forum Thumb. (Ⅱ). Effects of the Medium Constituent, Photoperiod and pH Value on the Growth, Differentiation and Chlorophyll Formation of Callus

百合癒合組織極易自其鱗片誘導，並能行繼代培養於改良之Murashige and Skoog培養基。培養基成分能影響癒合組織的生長與分化。培養基裡缺少植物荷爾蒙並未影響癒合組織的生長，但明顯地減弱分化芽條的能力，一般添力1 ppm NAA和1 ppm Kinetin時癒合組織生長最好，添加3 ppm Kinetin和1 ppm NAA對芽條分化較好，NAA超過3 ppm則癒合組織之生長和分化皆顯著受到抑制，尤其是NAA濃度超過5ppm時，癒合組織生長完全受到抑制。癒合組織之生長隨著椰子汁加入量而遞增，但芽條分化率則相對減低，培養基內缺少維生素及myo-inositol並未影響百合癒合組織的生長。12小時之光週期對百合癒合組織之生長及分化最好，24小時連續照光並不能促進癒合組織生長，反而抑制分化能力，連續黑暗對癒合組織生長與分化皆有不良影響。低濃度之NAA和Kinetin及適當之蔗糖可促進百合癒合組織葉綠素之生成，但NAA超過4 ppm或Kinetin超過9 ppm，則葉綠素之生成顯然受到抑制，培養其缺糖或pH值低於4.5對葉綠素生成及癒合組織生長，分化皆有不良影響。綜合而言，百合癒合組織在含有1 ppm Kinetin和1 ppm NAA, pH 5.5之培養基上，及12小時光週期下培養，生長最好，且能繼續分化幼苗。

The callus of Lilium longilorum Thunb was easily induced from scale sections and subcultured on a modified Murashige and Skoog medium. The constituents of medium affected the growth and differentiation of callus tissue Lack of plant hormone in the medium did not affect callus growth, but significantly reduced the differentiation of bud. When the medium contained 1 ppm each of NAA and kinetin, the callus grew and differentiated well. When the medium contained 3 ppm of kinetin and 1 ppm of NAA, differentiation of shoot was the best. However, at the concentration of 3 ppm, NAAL suppressed the growth and differentiation of callus; NAA above 5 ppm, callus growth was completely inhibited. The callus grew well when coconut milk was added, but differentiation of shdot was reduced. Lack of vitamin B1 and myo-Inositol in the medium did not affect the growth. The callus of lilium grew well under a 12-hr light illumination and continuous light illumination, but the growth was considerably inhibited under dark condition. Chlorophyll formation was found in a large amount when NAA and kinetin were at the right condition. Above 4 ppm of NAA or 9 ppm of kinetin, chlorophyll formation was remarkably suppressed. In additidn, when the pH value was at 4.5 or 7.5 chlorophyll formation was decreased. In conclusion, the callus of lilium grew and differentiated very well when medium contained 1 ppm each of kinetin and of NAA, at pH 5.5 and under 12-hr light illuminatien of photoperiod.