DPX-1840和Chlorflurenol對於菸草癒合組織之生長及再分化的影響

Effect of DPX-1840 and Chlorflurenol on Growth and Redifferentiation of Tabacco Callus in vitro

DPX-1840和Chlorflurenol以1-10mg/e之濃度加入含0.6mg/e kinetin和10mg/e IAA的MS培養基中，抑制菸草（Nicotiana tobacum cv萬國士）瘉合組織的生長和不定根的發生。但是，若以1-10mg/e之濃度的DPX-1840和Chlorflurenol加入含3mg/e kinetin 的MS培養基中卻能促進瘉合組織產生不定芽數2~4倍。文中並討論抑制auxin運移的特性和促進不定芽發生的可能原因。

The auxin transport inhibitors, DPX-1840 (3,3a-dihydro-2(p-methoxy-phenyl)-8H-pyrazolo (5,1a) isoindol-8-one) and chlorflurenol (2-chloro-9-hydroxyfluorene-9-carboxylic acid), at concentration of 1-10 mg/e, inhibit the growth of callus derived from the pith parenchyma of Nicotiana tobacum cv 萬國士. On the other hand, under the same concentrations, these auxin transport inhibitors promote 2-4 flods shoot formation in presenee of kinetin (3 mg/e). The possible role of these compounds on growth and redifferentisation of tobacco callus is dicussed.