利用花粉遺傳標誌區別柑桔有性胚

Identification of Sexual Embryo by Genetic Marker in Polyembryonic Citrus

以白色胚之晚白柚和蜜柚花粉，分別授粉於黃綠色胚之四季桔，果實於即將成熟時採收，檢查種子之胚數與胚色。以蜜柚授粉者平均胚數4.1，以晚白柚授粉者2.4，胚色區分為乳白色、黃綠色及介於兩者間之淡黃綠色？每一雜交種子中至多出現一個乳白色胚，為有性胚，其所萌發之幼苗皆具有柚類之大型葉片；黃綠色胚所萌發之幼苗，其特徵與四季桔相同，為珠心胚；淡黃綠色胚甚少，成活的五株中，四株為有性胚。

Calamondin (C. madurensis Lour.) was pollinated with two varieties of pummelo (C grandis (Linn.) Osbeck), ”Mi-youh” and ”Wan-pai-youh” Xenia phenomenon was found In hybrid seeds, and zygotic embryos were identified by its colour. In nine month old, all the seedlings germinated from cream embryos, which have. large leaves are zygotic,. and nucellar seedlings germinated from yellow green. embryos. Among the, five survived seedlings germinating from pale yellow green embryos, four of them are zygotic and the other one is nucellar.