英文摘要 |
The papaya cuttings, propagated from lateral shoot with 2,000 ppm IBA treatment for rooting, were grown in September to elucidate the plant characteristics, fruit yield and quality. Field cutting plants have been characterized by excessive vegetation within 126 days after planting and reduced juvenile stage for 133 days. Young trees have grown vigorously and set flower and fruit at 33 cm above ground or 63 days after planting. Seedling, when planted in this environment in September, have a juvenile section of 115 cm high before flowering. Daughter plants are same of the sex and should also have the same fruit of excellent quality as the parent plants from which the cuttings are taken. Comparied with autumn or winter season, parent trees can be cut back in summer to encourage lateral shoot production and hasten root formation easily. However, only a total of 24 lateral shoots can be taken from parent tree per year. The microcuttings on the other hand, obtained from 1.5~2.0 cm shoot tip of tissue culture multiple shoot with 0.5 ppm IBA treatment for rooting, were also grown in September and have the same plant growth characteristics with higher fruit yield as cutting. The micropropagation effeciency of papaya was the best on this studies than before. The primary multiple shoot in vitro 6 cm high, cultured from apics of field growing plant on transplanting medium, were dissected into double-node segment and culture for 3 weeks on multiplication medium. 5~7 multiple shoots that have developed from segment were dissected into double-node segment and culture on the multiplication medium again, giving a multiplication rate of 6 X 14n (n = no. of subculture) in 3 weeks. Meanwhile, the shoot tips 1.5~2.0 cm long can be cut from multiple shoot and cultured on rooting medium containing 0.5 ppm IBA for 3 weeks, giving a rooting percentage of 80% and multiplication rate of 2.4 X 14n in 3 weeks. Ultimately, the papaya microcuttings with tissue culture were more available than lateral cuttings and seedlings. |