利用複式聚合酵素鏈鎖反應檢驗抗嘉磷賽除草劑大豆加工製品及毛豆

Detection of Roundup Ready(superscript TM) Soybean Processed Food and Vegetable Soybean by Multiplex Polymerase Chain Reaction

基因改造食品之檢測於食品安全法規上有其必要性。國內基因改造食品標示事項，於2003年起實行強制標示。本研究是針對導入以抗嘉磷賽除草劑基因之大豆及以其為原料之加工品，包含黃豆、豆漿、豆腐、豆干、豆皮、素肌、大豆蛋白、素肉及毛豆等，以複式聚合酵素鏈鎖反應進行檢測，以尋求其檢測方法。結果顯示以此方法檢測時，黃豆、豆漿、豆腐、豆干及豆皮等可達5%以上之靈敏度，而素肌及大豆蛋白亦可做有效檢測；素肉因其DNA分子破壞過於嚴重，無法以此種方法檢測。另外目前國內生產之毛豆則皆未檢測出含有抗嘉磷賽除草劑大豆基因成分。

The detection of GM-food has its necessity due to food safety regulation, and the labeling of GM-food will be practiced by 2003 in Taiwan. In this study, products such as soyamilk, tofu, dried tofu, yaba, su-ju, soybean protein, vegetable meat and vegetable soybean made of Roundup Ready(superscript TM) Soybean (RRS) were detected by multiplex polymerase chain reaction. By this method, it showed a limit of detection for reference samples containing 5% (w/w) RRS of products soybean, soyamilk, tofu, dried tofy and yaba. This method can be used to detect su-ji and soybean protein efficiently but not in vegetable meat due to its DNA degradation. We also found that no RRS gene been detected in vegetable soybean harvested in Taiwan.