以不同型式生物反應器進行朵麗蝶蘭微體繁殖

Micropropagation of Doritaenopsis in Various Types of Bioreactors

朵麗蝶蘭類原球體分別以氣動壓差淹灌式、滾瓶、攪拌式、與氣舉式等不同型式之生物反應器大量培養，結果以氣動壓差淹灌式及滾瓶反應最好，攪拌式與氣舉式則不能適用。而氣動壓差淹灌式生物反應器中內鍋之設計型式與材質亦影響類原球體之增殖及分化。利用氣動壓差淹灌式生物反應器培養四種不同品種之朵麗蝶蘭類原球體，其增重倍率在42-65倍／月之間，依品種而異。每槽類原球體經培養一個月重量可達100g以上，並預估能產生3000-5000株小苗。而實驗顯示滾瓶培養之結果與氣動壓差淹灌式生物反應器相似，亦可做為朵麗蝶蘭或蝴蝶蘭以類原球體大量繁殖之另一種選擇。

Protocorm-like bodies (PLBs) of Doritaenopsis were cultivated in various types of bioreactors including air-driven periodic immersion (API) bioreactor, rolling bottle, spinner flask and airlift bioreactor. The best results were given by API bioreactor and rolling bottle among all tested bioreactors. Design and composition of inner container used in API bioreactor also affected proliferation and differentiation rate of PLBs. The proliferation rates of four different cultivars propagated in API bioreactor were ranged from 4.2-6.5 folds /month by weight. After one harvest, each API container could generate over 100 g of PLBs, which was estimated 3000-5000 plantlets at least. Our results indicate the increase of biomass from rolling bottle system is similar to the API bioreactor as well. Therefore, rolling bottle can be used for mass micropropagation of PLBs on Doritaenopsis or Phalaenopsis as another choice beside API bioreactor.