利用體胚形成進行薑花之微體繁殖

Micropropagation of Hedychium coronarium Köenig via Somatic Embryogenesis

利用體胚形成模式進行薑花之微體繁殖，除了能夠在短時間內達到大量繁殖的目的外，更可利用作為基因轉殖之材料使用。葉鞘及葉培植體在含5 mg/l 2，4-D，0.5 mg/1 BA、3%蔗糖及200 ml/1椰子水的VW固體培養基中，經3個月，可誘導產生具體胚分化能力之癒合組織；並於含4 ms/l BA及0.05 ms/l NAA之MS培養基中，直接由此癒合組織的表面產生數個至數十個數目不等之體胚，2個月後可形成一完整植株。配合0.1M山梨糖醇處理可明顯的改變組織質地，並些微提高癒合組織之體胚形成率。

The purpose of this experiment using somatic embryogenesis was not only to develop the micropropagation method of ginger lily in a short period of time, but expected to be used for the materials of future transgenic plant. Among different organ explants from ginger lily, only sheaths and leaves cultured in VW medium containing 5mg/l 2, 4-D、0.5mg/l BA、3% sucrose and 200ml/l coconut water for 3 months could be induced to form embryogenic callus. It could produce lots of germinated or ungerminated somatic embryos per culture directly from the surface of calli after culture in MS medium containing 4mg/l BA and 0.05mg/l NAA. The shoots could develop into intact plants with vigorous appearance after 2 months in culture. The amplified callus showed slightly increased embryogenesis frequency after being exposed to an osmotic stress by sorbitol.