果莢成熟度、培養基組成分與液體培養對芭菲爾鞋蘭屬之Paphiopedilum primulinum種子發芽之影響

Effect of Capsual Maturity, Medium Composition and Suspension Culture on in vitro Germination of Paphiopedilum primulinum Seed

以Paphiopedilumprimulinum授粉後110天的綠果莢播種，8星期後可達52.4％的發芽率，授粉後130-150天者則發芽率降至30.8-34.2%。低鹽類濃度1/4-1/8MS較1/2MS有利於種子發芽。鹽類組成分以ThomaleGD配方與1/4MS發芽最為理想，分別為58.6%與56.0%。培養基中添加椰子水對種子發芽有顯著的促進效果，馬鈴薯泥與tryptone次之，香蕉泥則有抑制發芽的作用。添加活性碳有利於種子發芽後原球體的發育。液體懸浮培養比固體培養有較高的發芽率，並且發芽速度較快而整齊。

The optimal seed germination of Paph. primulinum in vitro was 110 days after pollination. Seed germinated well in 1/4 MS, 1/8 MS and Thomale GD media. Among the organic supplements tested, coconut milk I 00 ml亡appeared to be the best for stimulating germination, followed by 20 g亡potato homogenate and 2 g·L·'tryptone, while 20 g·L·1 banana homogenate was shown to have a negative effect The addition of activated charcoal seemed to have a little effect on germination percentage, but promoted further development of the protocorms. Suspension culture resulted in a faster, more uniform and higher germination rate, as compared with solid culture.