利用不定芽再生進行綬草微體繁殖

Micropropagation of Spiranthes sinensis (Pers.) Ames Using Adventitious Shoot Regeneration

綬草Spiranthes sinensis（Pers.）Ames授粉後10-12天的未成熟種子無菌播種於含花寶1號3g/L.半量MS維生素，peptone 2g/L、蔗糖lOg/L及agar 8g/L的培養基中，一個月後約有70-80%的發芽率。以幼嫩實生苗為培植體，培養在以花寶1號為基本鹽類加BA 1-Smg/L的固態培養基內，4個月內可增殖4-6倍之不定芽。成熟植株去頂後以BA 3-Smg/L培養，2個月的增殖率約為3倍。不定芽分切後移至不含任何植物生長激素的培養基即可發根，在添加100ml/L椰子水的培養基內，其發根率可達100%。本研究顯示綬草可應用不定芽的方式進行增殖，以達大量繁殖之目的。

Ten-to twelve-day-old immature seeds of Spiranthes sinensis were germinated in vitro on medium containing Hyponex #I 3g/L, half strength MS vitamins, peptone 2g/L, sucrose IOg/L and agar 8g/L Germination rate was 70-80% after one month culture. Adventitious shoots regenerated from young seedlings were increased 4-6 times on Hyponex # I medium with BA 1-5 mg/L in 4 months. Three fold of proliferation rate were obtained from wounded shoot tip of mature plant supplemented with BA 3-5 mg/L at two-month intervals. Adventitious shoots were separated and transferred onto rooting medium without any plant growth regulator. Rooting rate of regenerated plantlets reached I 00% in addition of I 00ml/L coconut water. These results suggested that adventitious shoot could be used on a large scale for micropropagation of S. sinensis.