玫瑰花微體繁殖補充二氧化碳於培養容器內之方法

In Vitro Carbon Dioxide Enrichment for Rose Micropropagation

在70µmol·m-2·sec·1的光強度下，培養現代薔薇（Rosa hybrida Hort.）'Samantha'或'Landora'培殖體的容器內，匱接通入2000ppm的二氧化碳15分鐘。在停止通氣後75分鐘，'Samantha'培養在培養基含蔗糖濃度為15g/ I或7.Sg/1,其培養容器內二氧化碳濃度分別為223ppm或211ppm：培養'Landora'的培養容器內二氧化碳濃度則分別為316ppm或1 40ppm。對於'Samantha'或'Landora'培殖體，於明期開始後2小時起，以每隔兩小時通入2000ppm的二氧化碳於培養容器內15分鐘，在7.5g/l低蔗糖濃度培養基培養的培殖體，其乾物重最重，外觀生長情況也最好。取自原來培養在補充二氧化碳的容器內的'Samantha'和'Landora'微體插穗，扞插於噴霧床三週後，微體插穗的根系發育較原來培養在未補充二氧化碳者佳。又'Landora'的培殖體培養在含7.Sg/1蔗糖之培養基配合二氧化碳處理者，扞插三週後其側根較原來培養在含蔗糖！Sg/1之培養基者多。文後並討論在組織培養以補充二氧化碳馴化培殖體之方法。

Under 70µmol·m·2·sec·1 photosynthetic photon flux density(PPFD), explants of Rosa hybrida Hort. cvs Samantha and Landora were cultured in containers enriched with carbon dioxide of 2000 ppm for 15 min. After 75min, the concentration of carbon dioxide in the contaier was 223 or 211 ppm, respectively, when 'Samantha'explants were cultured on the medium containing I 5g/l or 7 .5g/l sucrose. 'Landora'explants were cultured on the same medium above, the concentration of carbon dioxide in the container was 316 or l 40ppm respectively. Under 70µmol·m·2·sec·1 PPFD, for 2 hours, culture containers were pumped carbon dioxide of 2000 ppm·for 15 min at the beginning of every 2 hours. The exp Ian ts of'Samantha'and 'Landora'had the heaviest dry weight and the best performances when they were cultured on the medium containing 7.5g/l sucrose. Microcuttings were inserted under mist for 3 weeks. Microcuttings of'Samantha'and 'Landora'from containers enriched carbon dioxide rooted well than those from containers without carbon dioxide enrichment. In addition, with carbon dioxide enrichment,'Landora' explants grown on the medium containing 7.5 g/1 sucrose were rooted well than those grown on the medium containing 15 g/1 sucrose; the former microcuttings had more lateral roots. The method of carbon dioxide enrichment for acclimatization in vitro was also discussed .