蔗糖對玫瑰花組織培養培殖體光自營生長的影響

Effect of Sucrose on the Photoautotrophy of Rose Explants in Vitro

玫瑰花組織培養枝梢培殖體的生長，會受培養基中所含蔗糖濃度的影響。‘Samantha’和‘Landora’的培殖體培養在蔗糖濃度為30g／l者，培殖體鮮重和乾重生長量最高；其次為培養在蔗糖濃度為15g／l者；再其次為培養在蔗糖濃為7.5和3.8g／l者。‘Pitica’的培殖體，以培養在蔗糖15g／l培養基的培殖體，其鮮重和乾重生長量最大；而以培養在蔗糖度為3.8g／l者生長量最小。三品種培殖體以培養在含蔗糖15g／l或30g／l培養基者增殖率較高。經四週培養結束時，其明期的二氧化碳的平衡點很明顯的分成兩組；培養於較低蔗糖濃度之培養基者，二氧化碳的平衡濃度降低至290ppm以下，而培養於高蔗糖濃度（30g／l）之培養基者，容器內二氧化碳的平衡濃度則在573ppm以上。而在暗期，容器內二氧化碳的平衡濃度則隨培養基之蔗糖濃度增加而增加。

The growth of rose shoot explants in vitro was affected by the sucrose concentration in the medium. When the 'Samantha' and 'Landora' explants were cultured on a medium with 30g/l of sucrose, the fresh and dry weight were the heaviest followed by the one which was cultured on a medium with 15g/l of sucrose. The poorest growth was when the explant was cultured on a medium with 3.8g/l of sucrose. The 'Pitica' explant showed the highest growth when it was cultured on a medium with 15g/l of sucrose, and the lowest growth was on a medium with 3.8g/l of sucrose. The propagation rates of these 3 cultivars were higher when they were cultured on a medium with 15 or 30g/l of sucrose. After 4 weeks in culture, the balance level of CO2 during the light period was apparently divided into 2 groups. The balance level of CO2 in the one cultured on a medium with lower level of sucrose dropped to below 290 ppm, while that in the one cultured on a medium with higher sucrose level (30g/l) was at 573 ppm. Furthermore, during the dark period, the balance level of CO2 in vessel increased when the level of sucrose increased.