利用AFLP分子標誌技術探討台灣原生種青脆枝族群之遺傳變異

opulation Genetic Variation of Nothapodytes foetida (Wight) Sleumer in Taiwan Base on Amplified Fragment­Length Polymorphism

本研究利用增殖片段長度多型性（AmplifiedFragment­LengthPolymorphism，AFLP）方法評估青脆枝（Nothapodytesfoetida（Wight）Sleumer）的遺傳變異。於蘭嶼、綠島、台東、屏東、高雄及嘉義等地區採集8個族群64個樣品，共測試64個引子，其中8組引子可擴增114個核酸條帶，101個多型性條帶。經由分子變異分析（analysisof molecular variance，AMOVA），地區間變異佔總變異的69.55%，地區內的變異佔總變異30.45%，顯示青脆枝族群於地理區域間變異大於地理區域內之變異，地區間有明顯分化現象。經由遺傳距離的群集分析及主座標分析結果，可在目似度0.80處將青脆枝分為2大族群，第II群為採自路竹地區之樣品，第二群中採自蘭嶼者形成單獨之群落為I­1群，其餘位於知本、綠島、九如、新港、里港、中埔等地區的青脆枝為第I­2群。於相似度0.87處，可再將第I­2群分為4小群。此2大族群內樣品之間的相似度雖可高達0.90以上，但是地理距離接近族群，於歸群樹狀圖並未先歸成一群，顯示台灣本島栽培種間之差異與地理隔離無明顯相關性，可能與人為栽種有關。 The research is to investigate the genetic variation of Nothapodytes foetida (Wight) Sleumer populations through amplified fragment length polymorphism (AFLP) molecular markers technique. The 8 populations were collected from Lanyu Island, Lyudao Island, Chihpen (Taidon County), Jiouru and Ligang (Pindon County), Lujhu (Kaohsiung County) and Singang and Jhongpu (Chiayi County) respectively. There were 101 polymorphic bands generated by 8 selected AFLP primer pairs. Based on the analysis of molecular variance (AMOVA), the genetic variance among N. foetida populations (69.55%) was higher than within the populations (30.45%). The differentiations among N. foetida populations were significant. Similar results were obtained through unweighted pair group method analysis (UPGMA) and principal coordinate analysis (PCA), dividing N. foetida populations into two groups in 0.8 similarity. The population of Lujhu was classified as group II, the population of Lanyu Island was classified as group I­1, the others were classified as group I­2. The gene pools constituted by the N. foetida samples collected from Luchu were significantly different with the native N. foetida from Lyudao Island and Lanyu Island. There existed some geographical genetic differentiation among cultivated N. foetida populations.