茄紅素、α-胡蘿蔔素與β-胡蘿蔔素高效液相層析法之建立

A High Performance Liquid Chromatography Method for Determining Lycopene, α-Carotene and β-Carotene

本試驗以HPLC方法，配合逆相C18管柱，探討移動相acetonitrile及methanol比例之不同對lycopene、α-carotene與β-carotene沖提效果之影響。分析結果顯示，移動相隨著methanol的比例增加，有利lycopene之分離並減少分析時間，然對於α-carotene與β-carotene分離則有降低之情形，因此以梯度之移動相沖提可得較佳之結果，並有良好之再現性與回收率。 The aim of this study is to elucidate the effect of raito of acetonitrile and methanol on separating lycopene, α-carotene and β-carotene by HPLC with RP-C18 column. Results showed that the three carotenoids were separated by isocratic mobile phase, the higher methanol ratio of isocratic, the less analysis time and better separation effect of lycopene. In constrast, higer methanol ratio led to the lower separation effect of α-carotene and β-carotene. Eluention with acetonitrile and methanol binary gradient mobile phase showed a better consistency and recovery in this research.