夜來香組織培養苗發根之研究

Rooting of In vitro Plantlets in Polianthes tuberosa L.

於修正過的MS基礎培養基中分別添加IBA或NAA誘導夜來香‘單瓣’及‘重瓣’品種瓶內不定根之發生，結果顯示低濃度處理即可有效的誘導不定根的發生，產生有正常功能的根毛，並促進瓶苗生長及提高出瓶存活率。但NAA濃度提高至1.0 mg／L時，促使根部呈塊狀畸形生長，TTC（2，3-5-triphenyltetrazolium chloride）還原反應增加，根數減少、根伸長受抑制，芽體葉綠素含量及葉片TTC還原力則較低，芽體的生長明顯受到抑制，推測此為造成瓶苗出瓶存活率低僅至28.2-34.2%之原因。而瓶內發根之苗株，出瓶存活率顯著較直接瓶外發根者為佳。 The modified MS medium supplemented with IBA or NAA was used to induce adventitious root formation of Polianthes tuberosa｀Single＇ and｀Double＇. Low concentration of IBA or NAA could induce root formation effectively, and produced normal functional root hair to improve the growth of plantlets in vitro and the survival rate of plantlets after transplanting. However, NAA concentration at 1.0 mg/L showed massive deformity root growth, increased TTC reduction reaction, decreased number of roots, and inhibited of root elongation. The chlorophyll content of shoot and TTC reduction ability of leaf was low. Therefore, shoot growth was significantly inhibited. It was supported that low survival rate (28.2-34.2%) of rooted plantlets was influenced by high concentration of NAA. The survival rate of in vitro rooted plantlets was higher than those of in vitro plantlets without root.