組織培養星辰花玻璃質化之影響因子

Factors Effecting Vitrification of Tissue-cultured Statice

本實驗以星辰花組織培養繁殖的芽體為試驗材料，探討不同培養基組成與型式、透氣度各異的培養盒，對試管苗玻璃質化與增殖率造成的影響。結果顯示，含有洋菜的培養基，不論培養盒的型式，都比含有水晶洋菜的培養基，在相同處理下，可以有效降低玻璃質化培植體的發生，提高芽體增殖率。L型培養盒及封石蠟膜的GA-7培養盒有較高的玻璃質化比率和較低的增殖率，可能與容器透氣性有關。而D型與未封石蠟膜的GA-7培養盒具有相似的培養效果，較適宜作為星辰花的培養盒。C8（BA 1.1 mg／l，NAA 0.5 mg／l）培養基比C1（IAA 4.0 mg／l，kinetin 2.5 mg／l）培養基有較高的增殖率，但亦有較高的玻璃質化植株比例。 The aim of this study is to investigate the combined effects of medium composition, size, shape and ventilation of various vessels on the degree of vitrification and proliferation of tissue-cultured statice. The results indicated that vitrification was effectively reduced and proliferation rate was improved on agar-solidified medium than medium with gelrite. Explants in GA-7 vessels with parafilm and L-type vessels had higher vitrification frequency and lower proliferation rate than other treatments. It may be related to poor ventilation of culture container. Treatments in D-type and GA-7 vessel without parafilm showed similar results, which is suitable for statice micropropagation. More shoots were regenerated on C8 (BA 1.1 mg/l, NAA 0.5 mg/l) than on C1 (IAA 4.0 mg/l, kinetin 2.5 mg/l) medium, however, less vitrified explants were produced on C1 than C8.