出瓶馴化光度與培植期對‘綠巨人’白鶴芋組培苗出瓶生長之影響

Effects of Photosynthetic Photon Flux Density during ex vitro Acclimatization and Duration of in vitro Culture on the Growth of Micropropagated Spathiphyllum ‘Sensation’

‘綠巨人’白鶴芋組織培養苗出瓶置於25±1℃之育苗室，每日分別處理16小時之35-150µmol m-2 s-1光度馴化10-30天，並以未經馴化移植置於溫室者為對照組，結果顯示組織培養苗出瓶之適宜馴化光度為100-150µmol m-2 s-1，其成活率與生長表現均較佳。培植4及14週者出瓶後植株生長較差，以培植6-8週培殖體具有3-4片葉、乾重30-33 mg者較有利出瓶後生長，並可縮短組織培養至出貨時間。培殖體在出瓶移植至無土混合介質後，以葉綠素螢光儀測定瓶內形成葉片之葉綠素螢光值（Fv／Fm），移植後第三天各處理葉片Fv／Fm值呈現下降之趨勢，並於馴化第7天後葉片Fv／Fm值開始回升，至馴化第15-30天，其值為0.78。 Spathiphyllum ‘Sensation’ plantlets were transplanted and placed in a growth room at a temperature of 25±1℃ under cool-white fluorescent light providing 16 h daily photosynthetic photon flux density of 35-150 µmol m-1s -1. After 10 to 30 days of acclimatization in the growth room, the plantlets were transferred to the greenhouse. The optimal irradiances ranged from 100 to 150 µmol m-2 s-1 during acclimatization. The 6 to 8-week-old plantlets in vitro, with 3 to 4 leaves and 30-33 mg dry weights, had greater subsequent ex vitro growth than did the 4 or 14-week-old plantlets. All the Fv/Fm values of in vitro formed leaves decreased on day 3, the value started to increase from day 7 and the Fv/Fm remained at 0.78 from day 15 to 30 after transplanting to soilless mix.