綬草無菌播種與生長發育

In vitro Seed Germination and Plant Development of Spiranthes sinensis （Pers.）

綬草（Spiranthes sinensis （Pers.） Ames）蒴果消毒後，將種子播在添加20 g/l蔗糖、50 g/l香蕉泥、1g/l活性碳的1/4 MS基礎培養基，一個月後，形成原球體。培養五個月後，原球體長成葉長約2公分、根2-3條的幼苗，但其中有13.4%為白化苗。將綠葉幼苗繼代到添加20 g/l蔗糖、30 g/l馬鈴薯泥、1g/l活性碳、150 ml/l椰子水的1/4 MS基礎培養基，再經二個半月後，幼苗可長到5公分長。幼苗經馴化後移植出瓶，97%的小苗可存活。且在四個月後，65%小苗抽苔開花，花色如同母株為粉紅色，但有2.5%為白花植株。此法可在一年之內完成綬草從種子發芽到成苗開花的生活史。 The capsules of Spiranthes sinensis (Pers.) Ames. were sterilized by diluted sodium hypochloritesolution and the seeds were sown in the medium containing 1/4 strength of MS basal medium supplementedwith 20 g/l sucrose, 50 g/l banana pulp and 1 g/l activated charcoal. Green protocorms were then observedafter one month. The seedlings were grown to plantlets with shoot and root after 5 months of culture. Theplantlets were then subcultured onto the medium containing 1/4 strength of MS salt basal medium, 20 g/lsucrose, 30 g/l potato pulp, 1 g/l activated charcoal and 150 ml/l coconut milk. After 75 days of culture, theseedling (about 5 cm in height) were transplanted into a greenhouse with 97% survival rate. After 4 monthsof growing, 65% acclimatized plantlets flowered. The complete procedure of propagation the spiranthesorchids took less one year from seed germination to flowering.