Thidiazuron及2,4-D或Picloram促進火鶴花試管苗葉片之培養再生不定芽

Thidiazuron Promotes Adventitious Shoot Regeneration on 2,4-D or Picloram Containing Medium in in vitro Lamina Culture of Anthurium

火鶴花Anthurium sp. T2營養系試管苗葉片，以半量之Murashige及Skoog配方（1／2 MS）為基本培養基，單獨添加生長素Picloram或2，4-D不易再生芽體，但有少量的癒合組織形成；添加0.5 mg／l TDZ於2，4-D（0～0.5 mg／l）培養基，不定芽形成率介於10～30%；不論在1／2 MS或MS培養基，添加TDZ均能誘導癒合組織形成。T18營養系試管苗葉片培養於以全量MS及0.5 mg／l TDZ為基本培養基，添加Picloram之處理，易形成癒合組織，而不定芽形成率均較含單獨TDZ者低；再生之植株其外觀正常。 A method of plant regeneration from in vitro lamina culture of Anthurium by supplementing thidiazuron with either 2,4-D or picloram is examined. Whole leaf blade of T2 lamina produced few callus on 1/2 MS medium supplemented with 2,4-D or picloram. A combination of 2,4-D (0.1~1mg/l) and TDZ (0.5mg/l) led to 10~30 % of the explants regenerating adventitious shoots. MS medium supplemented with 0.5 mg/l TDZ and picloram (0.1~1mg/l) induced 0~70% shoot formation in T18 lamina. Reducing salt strength to one half promoted callus formation. Normal and rooted plantlets were recovered and subcultured on the same medium