長果桑芽體組織培養之研究

Tissue Culture of Morus laevigata Wall using Axillary Buds

取長果桑（Morus laevigata Wall）的側芽為培殖體，培養在含有MS鹽類基本培養基，1 mg／L BA及0.05 mg／L NAA中，經1個月照光培養，可長成具有2-4節間的芽梢，將芽體切下繼代增殖，在含有2 mg／L benzyladenine（BA）之培養基下，培殖體鮮重增加倍率最高，培養40天後可長出6.5支芽梢；增殖時添加活性碳無法抑制癒傷組織產生，反而抑制芽體增殖。不論增殖或發根，蔗糖濃度以30 g／L為最佳。培殖體於含有半量的MS培養基，0.5 mg／L indole-3-butyric acid（IBA）及30 g／L蔗糖下發根率可達100%，平均根長5.9公分，根數約10.1根，瓶苗在田間生育良好，成活率達90%以上。 Nodal explants obtained from five-month-old axillary buds were used to establish an in vitro propagation system of Morus laevigata Wall. Modified Murashige and Skoog (MS) medium supplemented with 30 g/L sucrose and 2 mg/L benzyl adenine resulted in a maximum rate of shoot proliferation (average of 6.5-shoots in 40 days). Adding activated charcoal did not inhibit callus formation but inhibited shoot multiplication. Sucrose concentration significantly influenced index fresh weight increase, root number, and survival rate of explants. A high frequency rooting was achieved using half-strength modified MS medium supplemented with 0.5 mg/L IBA and 30 g/L sucrose. The average root length and root number were 5.9 cm and 10.1, respectively. Well-developed plantlets were transferred to plastic pots with a survival rate over 90%.