以RAPD及NBS專一性引子擴增之DNA標誌探討芒果品種間之遺傳相似度

Genetic Similarity Analysis among Mangifera indica Cultivars / Lines Based on DNA Markers Amplified by RAPD and Specific NBS Primer

本研究以抗病基因中最主要之核苷酸結合富含白胺酸重複序列（nucleotide binding leucine-rich repeat, NBS-LRR）類型為分析對象，利用15對randomly amplifiedpolymorphic DNA（RAPD）引子與NBS專一引子組合，對32個芒果品種／系之DNA進行polymerase chain reaction（PCR），擴增出含有NBS區域之DNA序列，得到83個抗病基因區域之多型性條帶。經unweighted pair-group method using arithmetic averages（UPGMA）及neighbor joining（NJ）分析，32個品種／系NBS類抗病基因型之遺傳相似度介於0.64-0.84間，可分為5大群，作為芒果NBS類抗病基因型分類的依據。本試驗結果所得之專一性分子標誌可提供芒果栽培品種鑑別之工具。 The objective of this study was to analyze the nucleotide binding leucine-rich repeat (NBS-LRR) in analog resistance gene family in Mangifera indica using randomly amplified polymorphic DNA (RAPD) markers. The combinations of 15 RAPD primers and 1 NBS specific primer were used to amplify NBS sequences complementary to the DNA sequences from 32 cultivars / lines. Eighty-three polymorphic bands of the NBS-LRR resistance gene analogs were obtained. The data were then analyzed by unweighted pair-group method using arithmetic averages (UPGMA) and neighbor joining (NJ) programs. Five groups were clustered ranging from 0.64 to 0.84 of similarity coefficient, indicating that NBS might be used for phylogeny of the analog resistant gene in mango. Some specific polymorphic bands obtained in this study could be used for mango cultivar identifications.