施用Harpin與Chitosan誘抗劑對提昇蝴蝶蘭貯運後品質之效果

Effects of Harpin and Chitosan Elicitors on the Improvement of Post-shipping Quality of Phalaenopsis Orchids

本研究旨在應用harpin及chitosan二種誘抗劑，以期改善蝴蝶蘭海運後之品質。結果在模擬貯運前施用5g•L^(-1)harpin或10mL•L^(-1)chitosan於蝴蝶蘭葉片，經三至四週之模擬黑暗貯運試驗後，harpin處理組減低蝴蝶蘭黃葉病的比率，而chitosan處理組則未減低感病。在接種鐮胞菌（Fusarium solani）的試驗中，harpin和chitosan皆可減緩蝴蝶蘭植株對鐮胞菌之感病病徵。以西方點墨法（Western blot）分析顯示，harpin及chitosan處理可誘導pathogenesis-related protein 2（PR2）蛋白質累積；經模擬貯運後，harpin處理組蝴蝶蘭組織中PR2蛋白顯著增加，而chitosan處理組則PR2蛋白降低。Harpin和chitosan皆可誘導蝴蝶蘭PR基因的表達，而增加植株抗病性。Harpin可誘導持續性抗病性，降低蝴蝶蘭在貯運過程中的感病率；然而chitosan雖可誘導蝴蝶抗病性，但無法降低貯運過程中的感病率。 The effectiveness of harpin and chitosan treatments on the improvement of the quality of Phalaenopsis plants after simulated long-distance shipping was investigated. Harpin and chitosan were applied at 5 g•L^(-1) and 10 mL•L^(-1), respectively, before 3-4 weeks of simulated shipping. Harpin reduced the number of disease infected plants after the shipping, but chitosan did not. In Phalaenopsis plants inoculated with Fusarium solani, both harpin and chitosan treatments reduced the infectious disease symptoms. Western blot analysis indicated that pathogenesis-related protein 2 (PR2) was induced in both harpin and chitosan treatments. Harpin resulted in higher levels of PR2 accumulation than chitosan did. The latter reduced to an invisible level after the simulated shiping. It seemed that harpin might be effective on inducing a long-lasting disease resistance during long-distance shipping of Phalaenopsis plants.