Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis

Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon(PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimatemetabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA toform BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, andteratogenicity. In this study, we employed our developed LC-MS/MS method to detect andquantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and42 birth defect cases. We determined that there is no significant difference in the level ofBPDE-dG formation between the normal and birth defect groups. This represents the firsttime to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples.The results indicated that under experimental conditions, BPDE-dG adducts were detectedin all the human umbilical cord blood samples from the normal and birth defect groups.