| 英文摘要 |
Vitamin D has been considered to regulate calcium and phosphorus homeostasis and topreserve skeletal integrity. Serum 25-hydroxyvitamin D (25(OH)D) is the best indicator ofvitamin D levels. The association of serum 25(OH)D deficiency with increased risk of type 1diabetes (T1DM) and type 2 diabetes (T2DM) is controversial. We investigated serum 25(OH)D2 and 25(OH)D3 levels in diabetes patients by using liquid chromatography tandem massspectrometry (LC-MS/MS). Serum 25(OH)D2 and 25(OH)D3 levels were measured with liquidchromatography tandem mass spectrometry in electrospray ionization positive mode.Chromatograms were separated using an ACE5 C18 column on a gradient of methanol. Thetotal 25(OH)D levels were calculated as the sum of 25(OH)D3 and 25(OH)D2 levels. A total of56 patients with T1DM and 41 patients with T2DM were enrolled in this study. There were42 and 28 non-diabetic, age-matched volunteers who participated as the T1DM controlsand the T2DM controls, respectively. The total 25(OH)D levels were lowest in the 21e40 agegroup. The levels of both 25(OH)D3 and the total 25(OH)D were significantly higher in theT1DM and T2DM groups than in the controls (p < 0.01 in T1DM and p < 0.05 in T2DM group,respectively). The 25(OH)D2 levels were only significantly higher in T1DM patients than inthe controls. The percentages of vitamin D deficiency (total 25(OH)D less than 20 ng/mL) in the T1DM, T2DM, the T1DM controls and the T2DM controls were 7.1%, 0%, 14.3% and 3.6%,respectively. The percentages of vitamin D insufficiency (total 25(OH)D less than 30 ng/mL)in the T1DM, T2DM, the T1DM controls and the T2DM controls were 26.8%, 7.3%, 54.8% and17.9%, respectively. The percentages of vitamin D deficiency and insufficiency weresignificantly lower in the T1DM patients than in the T1DM controls (p < 0.01). In the presentstudy, both type 1 and type 2 diabetes patients had higher serum 25(OH)D levels and lowerpercentages of vitamin D deficiency/insufficiency. |
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