| 英文摘要 |
Mass spectrometry (MS) is a type of analysis used to determine what molecules make up asample, based on the mass spectrum that are created by the ions. Mass spectrometers areable to perform traditional target analyte identification and quantitation; however, theymay also be used within a clinical setting for the rapid identification of bacteria. Thecausative agent in sepsis is changed over time, and clinical decisions affecting the managementof infections are often based on the outcomes of bacterial identification. Therefore,it is essential that such identifications are performed quickly and interpretedcorrectly. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) massspectrometer is one of the most popular MS instruments used in biology, due to its rapidand precise identification of genus and species of an extensive range of Gram-negative and-positive bacteria. Microorganism identification by Mass spectrometry is based on identifyinga characteristic spectrum of each species and then matched with a large databasewithin the instrument. The present review gives a contemporary perspective on thechallenges and opportunities for bacterial identification as well as a written report of howtechnological innovation has advanced MS. Future clinical applications will also beaddressed, particularly the use of MALDI-TOF MS in the field of microbiology for theidentification and the analysis of antibiotic resistance. |
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