以反捲積法解析市售保健飲品中有機物之紫外光全譜與分子量大小

Using deconvolution Method to resolve UV spectrum and molecular weight cut-offs of commercial health drinks

幾種市售保健飲品之UV與分子量之圖譜，由於肉眼難以視別各別成份之貢獻，採用反捲積法（Deconvolution Method）及高斯（Gauss）函數進行擬合，可更深入及精準檢視進行定性分析，並與廠商提供的牛樟芝標準品進行比較，提供較細微及有意義的觀察。實驗結果顯示，牛樟芝粉末配製母液中之色度對顆粒徑之分析會產生干擾。牛樟芝溶於乙醇或水溶劑，扣除溶劑及鹽類影響的波峰，最大的三個波峰分別落在251－284 nm間；牛梓芝與三類市售保健商品，包括靈芝3項、冬蟲夏草3項及人蔘5項，各選一項進行反捲積法之合併圖形與原圖之相關係數高，並達統計顯著，在靈芝部分，以285、300與391 nm為主要波峰，冬蟲夏草部分，則是以268與279 nm為主，人蔘特徵則是259與268 nm，與牛樟芝的265 nm及280 nm處有較近的共同特徵。在原始分子量圖譜採反捲積法，牛樟芝在UV＝265 nm及UV＝280 nm，對應的主要分子量大小分別為2.8 kDa及3.2 kDa。三類保健飲品最大波峰之分子量位置（UV＝265 nm）為3－4.5 k Da，接近牛樟芝2.8 k Da。三類種保健飲品最大波峰之分子量位置（UV＝280 nm）為3－4.5 kDa，三種樣本最大波峰與牛樟芝萃取液最大波峰位置3.2 k Da相似。研究結果証實，UV及分子量圖譜可藉反捲積法（Deconvolution Method）及高斯（Gauss）函數有效分離成份，作為定性及後續定量之參考。 The diagram of UV and molecular weight cut-off of several health drinks in the market had a difficulty by naked eye to select the optimal location to understand the contribution of individual components using deconvolution method and Gauss function. After this transformation, a more detailed and accurate view could be provided to make the qualitative analysis and a comparison with the commercially available standard products of Antrodia camphorata, providing more subtle and meaningful observations on commercial products, including Ganoderma lucidum, Cordyceps sinensis and Ginseng. Based on the consideration of particle size of the powder of Antrodia camphorata dissolved into ethanol was diluted with 10,000 time by ethanol and pure water, whole samples should be filtered by 0.2 μm membrane filter. The stock solution of Antrodia camphorata diluted with 10,000 times in both solvents had three UV peaks ranged from 251 nm to 284 nm excluding the effect of anion ions by deconvolution method and Gauss function. High correlation coefficients and statistically significant were found on simulated diagram in comparison with original diagram. For Ganoderma, there had three UV peaks of 285, 300 and 391 nm. UV Components of 268 nm 279 nm existed in Cordyceps sinensis. Ginseng had UV characteristic of 259 and 268 nm. Regarding to the original diagram of molecular weight cut-offs (MWCOs) applied with deconvolution method, the dominant values at UV=265 nm and UV =280 nm were respectively 2.8 kDa and 3.2 kDa. MWCOs of typical samples taken from each kind of commercial health drinks (UV=265 nm) ranged from 3 kDa to 4.5 kDa, approaching 2.8 kDa of Ganoderma lucidum. Similar location of MWCOs for typical samples taken from each kind of commercial health drinks were found between at UV=280 nm and UV=265 nm; however, the major location at UV=280 nm was close to the proximity of 3.2 k Da of Ganoderma lucidum. This research provide an evidence to confirm the applicability of Deconvolution method and Gauss function on the analysis of UV and molecular weight diagrams, being as a qualitative and subsequently quantitative reference.