清潔劑應用在動物DNA遺傳物質的保存

Laundry Detergents as Animal Tissue Preservatives for DNA Analyses

本文測試保存在常溫下酒精中2個月到9年間的動物組織樣本的DNA衰退程度，以及幾種清潔劑在長時間、高溫環境下保存DNA組織樣本的效果，並討論酒精在現今動物DNA組織樣本保存上的優缺點，及其應用在DNA序列判讀等分子生物學研究技術上的限制。測試結果顯示，保存在95%酒精中的組織樣本其DNA結構在7年後會有明顯的衰退現象，以70%酒精保存DNA的效果亦相當差。而多種清潔劑在一定的時間內對於DNA物質的保存效果與99.5%的無水酒精相近，但並非所有的家用清潔劑都適用於DNA樣本保存。

Several brands of laundry detergents were tested as the preservatives for animal tissues in DNA analyses. Their DNA degradations were compared to those from the tissues preserved in ethanol and DNA preservation buffer, and from cryopreservation (immersion in liquid nitrogen). The results showed that the tissues preserved in 99.5% ethanol and DNA preservation buffer were as good as those from cryopreservation, but those in 70% ethanol degraded faster. Some (not all) of the detergents brands tested were found to be as good as those preserved in concentrated ethanol and DNA preservation buffer. Preservation in a detergent-based solution is an easy and economic way for tissue preservation in DNA analyses.