β-CD-AITC抗菌微粒製備條件的探討

Study on Preparation of the Antimicrobial β-CD-AITC Particles

近年來許多研究指出，天然的抗菌成分有抑制細菌、黴菌之功效，本研究運用微膠囊技術以β－環狀糊精包覆異硫氰酸烯丙酯（β-CD-AITC）的方法，製備具有揮發性抗菌成份之β-CD-AITC微粒。在製備β-CD-AITC微粒過程中探討攪拌加熱時間（1、2、3小時）、乾燥條件（35°C真空乾燥、75°C真空乾燥）、AITC包覆量及不同儲存環境（28°C、100％ RH；38°C、100％ RH；38°C、50％ RH）下AITC的釋放量。研究發現製備微粒過程中攪拌加熱時間對β-CD-AITC抗菌微粒的包覆量有影響，隨著攪拌加熱時間的增加，包覆量從28.22±1.25μL/g增加至37.95±1.23μL/g。另外乾燥方式也會影響β-CD-AITC微粒的包覆量，兩種乾燥條件中以35°C真空乾燥所製備之β-CD-AITC微粒的AITC包覆量（81.74±0.76μL/g）最多，且有顯著性差異（p<0.05）。35°C真空乾燥和75°C真空乾燥所製備之β-CD-AITC微粒，在28°C-100％ RH, 38°C-50％RH,和38°C-100％ RH的環境下儲存5天，發現兩者皆會隨著貯存時間的增加，包覆量及釋放率都有減少之趨勢。此具有揮發性抗菌成份之β-CD-AITC微粒可應用至食品包裝，來延長食品之保存期限。 This study was to prepare β-cyclodextrin (CD)-AITC particles by encapsulation method and to examine the inclusion amount of AITC in different processes, and the inclusion amount and release rate of AITC of particles during storage. The inclusion amount of AITC was increased from 28.22±1.25 μL/g to 37.95±1.23 μL/g when the heating time increased from 1 hour to 3 hours during mixing. Different temperatures during vacuum drying process were introduced to prepare the β-CD-AITC particles: 35°C-vacuum, and 75°C-vacuum. Between these two processes, the highest inclusion amount (81.74±0.76 μL/g) of AITC was obtained when particles dried at 35°C -vacuum condition and the value was significantly different (p<0.05). The inclusion amount and release rate of AITC of particles were examined during storage at different temperature and relative humidity (RH). The inclusion amount and release rate of AITC particles, processed from both conditions at 35°C-vacuum and 75°C-vacuum, had a tendency for reduction when particles stored at all three conditions (28°C-100% RH, 38°C-50% RH, and 38°C-100% RH) for up to 5 days. The antimicrobial activity of the β-CD-AITC particles will be further studied for its application in food packaging to extend the shelf life of packaged foods.

This study was to prepare β-cyclodextrin (CD)-AITC particles by encapsulation method and to examine the inclusion amount of AITC in different processes, and the inclusion amount and release rate of AITC of particles during storage. The inclusion amount of AITC was increased from 28.22±1.25 μL/g to 37.95±1.23 μL/g when the heating time increased from 1 hour to 3 hours during mixing. Different temperatures during vacuum drying process were introduced to prepare the β-CD-AITC particles: 35°C-vacuum, and 75°C-vacuum. Between these two processes, the highest inclusion amount (81.74±0.76 μL/g) of AITC was obtained when particles dried at 35°C -vacuum condition and the value was significantly different (p<0.05). The inclusion amount and release rate of AITC of particles were examined during storage at different temperature and relative humidity (RH). The inclusion amount and release rate of AITC particles, processed from both conditions at 35°C-vacuum and 75°C-vacuum, had a tendency for reduction when particles stored at all three conditions (28°C-100% RH, 38°C-50% RH, and 38°C-100% RH) for up to 5 days. The antimicrobial activity of the β-CD-AITC particles will be further studied for its application in food packaging to extend the shelf life of packaged foods.