A rapid and simple high-performance liquid chromatographyeUV method was developed for the separation and quantification of salbutamol, ractopamine, and clenbuterol in pork. A mixture of acetonitrileeformic acideammonium acetate was used as the mobile phase to separate three b-agonists on a C18 column with gradient. The effects of the addition of formic acid and ammonium acetate to mobile phases on the separation of b-agonists were investigated. These additives can greatly improve the resolution and sensitivity. Under the optimized chromatographic condition, this separation does not need extra sample preparation. Complete baseline separation of three b-agonists was achieved in < 20 minutes; the linear range is 0.2e50 mg/L with a correlation coefficient R2 value of > 0.99. Excellent method reproducibility was found by intra- and interday precisions with a relative standard deviation of < 3%. The detection limit (S/N=3) was found to be <0.05 mg/L; this method can be used for routine screening of the b-agonist residues in foods of animal origin before being identified by confirmatory methods.
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