蘭嶼產瀕危植物腰果楠之微體繁殖

In Vitro Micropropagation of Dehaasia incrassate (Jacks) Kosterm. - an Endangered Woody Plant of Orchid Island

腰果楠為產於蘭嶼的瀕危植物，本研究利用一年生種子苗的頂芽及含節莖段在1／2MS添加2.5mg／L BA及0.5mg／L NAA兩星期可誘導芽體產生，產生芽體的莖段先在添加0.5mg／L BA及0.15mg／L NAA的WPM基礎培養基四至六週，再加入添加0.5mg／L BA及0.3mg／L NAA液體培養基雙層培養六週，可促進芽體抽長，再移至添加0.5mg／L BA及0.1mg／L NAA的WPM基礎培養基可促進發根，最終移入不含生長調節劑的WPM培養基一年，植株長至5-8cm移至瓶外可正常生長，本研究利用微體繁殖作為腰果楠物種保育的途徑之一。 Dehaasia incrassate (Jacks) Kosterm. (Lauraceae) is an endangerd tree species of theOrchid Island, located in the southeast of Taiwan. The shoot tips and nodal segments derived from 1-yroldseedling were induced for bud sprouting in MS semi-solid medium with macroelements at halfstrength and supplemented 2.5 mg/L BA, 0.5 mg/L NAA for 2wk. To shoot elongation explant wascultured for 4-6wk in WPM solid medium supplemented with 0.5 mg/L BA and 0.15 mg/L NAA,followed by 6wk in double phase conditions (solid medium with a layer of liquid medium on the top)using the WPM supplemented with 0.5 mg/L BA 及0.3 mg/L NAA in the liquid phase. Shoot wastransfer to WPM solid medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA for rooting.Seedlings followed transfer to plant growth regular free WPM solid medium for 1yr and grew up to 5-8 cm, then transplant to the greenhouse. The aim of the study was to development micropropagationprocedures for this endangered species to facilitate conservation.

Dehaasia incrassate (Jacks) Kosterm. (Lauraceae) is an endangerd tree species of theOrchid Island, located in the southeast of Taiwan. The shoot tips and nodal segments derived from 1-yroldseedling were induced for bud sprouting in MS semi-solid medium with macroelements at halfstrength and supplemented 2.5 mg/L BA, 0.5 mg/L NAA for 2wk. To shoot elongation explant wascultured for 4-6wk in WPM solid medium supplemented with 0.5 mg/L BA and 0.15 mg/L NAA,followed by 6wk in double phase conditions (solid medium with a layer of liquid medium on the top)using the WPM supplemented with 0.5 mg/L BA 及0.3 mg/L NAA in the liquid phase. Shoot wastransfer to WPM solid medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA for rooting.Seedlings followed transfer to plant growth regular free WPM solid medium for 1yr and grew up to 5-8 cm, then transplant to the greenhouse. The aim of the study was to development micropropagationprocedures for this endangered species to facilitate conservation.