平胃散對吲哚美洒辛引起胃潰瘍之保護作用   全文下載

Gastroprotective Effects of Ping-Wei San on Indomethacin-Induced Gastric Ulcer

平胃散在傳統醫學中，屬應用於幫助消化的方劑。本研究探討平胃散是否可以降低??美洒辛誘導之胃潰瘍，及是否影響其抗發炎作用。平胃散以沸水煎煮後，湯液經冷凍乾燥得萃取物。平胃散水萃物（H-PWS）以高效液相層析儀，定量其指標成分－－甘草酸（glycyrrhizin） 及橙皮?（hesperidin），含量分別為38 及 19 mg/g。繼而利用??美洒辛誘導大鼠胃粘膜細胞損傷，以急性胃潰瘍評估H-PWS之胃保護作用。結果顯示：H-PWS具有劑量依存性，降低??美洒辛誘導之胃粘膜細胞死亡，且在200 mg/kg劑量下，可減少??美洒辛誘導之大鼠胃潰瘍。於脂多醣刺激大鼠巨噬細胞（RAW 264.7 cells）試驗中，H-PWS可增強??美洒辛抑制NO及PGE2釋放作用，且H-PWS（200 mg/kg）亦可以增強??美洒辛抑制大鼠足腫，及血清中的PGE2含量。基於上述結果，顯示H-PWS具有降低??美洒辛誘導之胃潰瘍，且不影響??美洒辛之抗發炎作用。推測H-PSW可以被開發為胃保護劑，或是合併?美洒辛使用，以降低其副作用。

Ping-Wei San (PWS), a Chinese medical decoction, was used to improve digestive absorption. This study was aimed to determine whether treatment with PWS reduce gastric ulceration induced by indomethacin and influencing its anti-inflammatory effect. PWS was extracted with boiling water and the decoction was freeze-dried into powder (H-PWS). The amounts of glycyrrhizin and hesperidin in H-PWS were analyzed by a HPLC method to be 38 and 19 mg/g, respectively. Co-administration of H-PWS resulted in a dose-dependent inhibition of indomethacin-induced gastric mucosal cell death. H-PWS (200 mg/kg) also alleviated the indomethacin-induced gastric ulcer in male Wistar rats in vivo. The inhibition effects of NO and PGE2 production were enhanced by indomethancin co-treatment with H-PWS from LPS-stimulated RAW 264.7 cells. H-PWS (200 mg/kg) also augmented the anti-inflammatory effect of indomethacin in 1% carrageen-induced paw edema. Moreover, H-PWS co-treatment with indomethacin produced a lower PGE2 level in serum than only indomethacin. Based on the results, co-treatment with H-PWS reduced the occurrence of indomethacin-induced gastric ulcers without affecting the anti-inflammatory effect of indomethacin. Therefore, we suggested that H-PWS can be developed as an anti-ulcer drug and H-PWS could be taken with indomethacin to decrease ulceration.